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极化荧光光度计

简要描述:1)采用滤光片式荧光技术;2)荧光素检测水平可精确低于1 pM;3)样品量Z小可达到500ul、80ul、25ul;4)可用于如NADH,NADPH,DNA,RNA,蛋白质,缩氨酸黄曲霉毒素,ATP,荧光标记底物,若丹明(一种红色荧光染料),雌激素,蛋白酶活性,酶分析,细胞间动态分析,氨基酸,维生素, 药物代谢物,类固醇,等等;或荧光极化测量等;

  • 产品型号:SE型
  • 厂商性质:经销商
  • 更新时间:2023-08-16
  • 浏览次数:2444

产品介绍

进口SE型极化荧光光度计


High Sensitivity - Picomolar

Quantitative results

Minimal sample volumes and preparation

Rapid measurments

Programable Data collection

Variable Temperature Sample chamber

Easy to change external filter holders

Standard System 360-700nm

Expanded System 250-850nm


The Fluorescence Polarization Systems provide a rapid and precise way to measure equilibrium binding and/or molecular degradation, with picomolar sensitivity, for a wide range of biological molecules. Fluorescence polarization takes advantage of the change in the speed of rotation that occurs when a fluorescent molecule binds to another molecule. The Systems are useful in performing immunoassays, determining antibody specificity and affinity, and providing protein-DNA and receptor-ligand binding measurements. Affinity binding is measured directly in solution, with each data point obtained in just a few seconds (6 seconds minimum). This simple, direct approach eliminates the need for any filtration, gel mobility shift assays, or immobilization techniques, with fluorescent labeling eliminating the use of radioisotopes


The Fluorescence Polarization Systems are stand alone, bench-top instruments that offer sensitive polarization measurement (requiring as little as 10fmol/mL of fluorescently labled sample), in a single-tube-format. The instruments use standard disposable glass cuvettes and their small footprint

maximizes precious bench space. The instruments have an onboard operating software that lets the user store up to 99 user-defined protocols and send data directly to a printer or your personal computer for analysis.


Fluorescence Polarization System features:

Expanded System - 250-850nm combination Quartz Halogen and Mercury Vapor Lamps

Polarizers - Glan-Taylor prisms with Extinction coefficient >10,000 from 280-900nm


Standard System - 360-700nm Quartz Halogen lamp

Polarizers - Film type with Extinction coefficient >10,000 from 400-700nm


Both Instruments feature:

Filters - Special fluorescein Interference filters (490nm Excit. and 525nm Emiss.)

Easy filter exchange for use with wide range of fluophores

Sample format - Single tube, Cell holders for 6mm (100µl) and 10mm (500µl)

(5mm (25µl) is optional

Precision - <2mP S.D. at 1nM fluorescein for 0.5mL sample

Sensitivity - Accurate to 10-11M fluorescein in polarization mode

and 10-13M fluorescein in intensity mode

Temperature Control - Variable from 5°C to 75°C in 1°C increments,±1°C

Read Time - Minimum 6 seconds, variable number of read cycles

Programmable Protocols - 99 user defined, password protected

Interfaces - RS232-C, DB25 parallel

Printer - External Thermal (parallel) 40 column

LxH - 20.5" x 14.75" x 9.25" (52.6x37.8x23.7cm)

Net Weight - 35lbs. (15.9kg)

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